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. 2022 Jun 30;12:922196. doi: 10.3389/fonc.2022.922196

Figure 2.

Figure 2

OSE induces autophagy and apoptosis in MDA-MB-231. (A) Cells were treated with OSE (1.2 mg/ml) for 48 hrs. Micrographs were captured at magnifications of 200X. (B) Induction of autophagy by OSE. Cells were treated with OSE (1.2 mg/ml) for 48 hrs, and cytoplasmic (LC3 I) and lipidized LC3 II accumulation was detected by Western blotting. (C) Activation of apoptosis by OSE. Cells were treated with OSE (0.8 and 1.2 mg/ml) for 48 hrs, and cleavage of procaspase-3 was examined by Western blotting. (D) Cells were treated without (0) or with OSE (0.8, 1.2 or 1.6 mg/ml) for 24 hours, and then Caspase 3/7 activity measured. (* denotes p<0.05, ** denotes p<0.01, and *** denotes p<0.001).