Figure 3.

OSE inhibits migration and downregulates activated FAK in MDA-MB-231 cells. (A, B) Cells were treated with OSE (1.2 mg/ml) and cell migration was assessed by wound healing assay. (C, D) Inhibition of cell migration in transwell migration assay. Images of crystal violet-stained migratory cells were taken 6 h post-treatment. Values are represented as mean ± SEM of distance migrated (n = 3 replicates) (*p < 0.05). (E) Downregulation of pFAK by OSE. Cells were treated with OSE (0.8 mg/ml) for 5, 10, 30 and 60 mins, and the phosphorylation level of FAK was determined by Western blotting. (F) Quantitation of three independent replicates plotted as mean of fold change (***p < 0.001).