Table 1.
IC50 values (μg/mL) of TMX-MCEs-ETH and W, in comparison to TMX-MCEs-CHL, on MCF-7, 4T1 and Vero cell-lines, after 24 h treatment (Tamoxifen is the positive control; Untreated cells are the negative control).
| Cell-lines /Formulation | MCF-7 |
4T1 |
Vero |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1:2 | 1:3 | 1:4 | 1:5 | 1:2 | 1:3 | 1:4 | 1:5 | 1:2 | 1:3 | 1:4 | 1:5 | |
| TMX | 12.02 ± 0.02∗∗∗ | 5.05 ± 0.01∗∗∗∗ | 11.22 ± 0.01∗∗∗ | |||||||||
| Negative Control | ˃100 | ˃100 | ˃100 | ˃100 | ˃100 | ˃100 | ˃100 | ˃100 | ˃100 | ˃100 | ˃100 | ˃100 |
| TMX -N. oculata-ETH | 16.98∗∗ ± 0.007 | 24.54∗∗ ± 0.02 | 26.3 ± 0.01 | 30.19 ± 0.02 | 18.62∗∗∗ ± 0.01 | 19.05∗ ± 0.01 | 21.37∗ ± 0.05 | 30.9 ± 0.05 | 25.11∗∗∗ ± 0.01 | 35.48∗ ± 0.02 | 60.25 ± 0.02 | 61.65 ± 0.02 |
| TMX- N. oculata-W | 15.84∗∗∗ ± 0.06 | 21.87∗∗ ± 0.05 | 30.19 ± 0.03 | 33.88 ± 0.06 | 30.9 ± 0.01 | 31.62 ± 0.02 | 38.01 ± 0.02 | 41.68 ± 0.01 | 56.23∗ ± 0.02 | 85.11 ± 0.01 | 87.09 ± 0.02 | ˃100 |
| TMX -N. oculata-CHL |
31.62∗ ± 0.02 |
36.3 ± 0.02 |
46.77 ± 0.05 |
53.7 ± 0.02 |
15.48∗∗∗ ± 0.06 |
17.78∗∗∗ ± 0.01 |
19.95∗∗ ± 0.05 |
22.38∗ ± 0.03 |
33.88∗∗ ± 0.01 |
47.86∗ ± 0.02 |
60.25 ± 0.02 |
66.06 ± 0.03 |
| TMX-T. suecica-ETH | 16.98∗∗ ± 0.01 | 21.37∗ ± 0.02 | 21.37∗ ± 0.01 | 33.11 ± 0.03 | 13.8∗∗∗∗ ± 0.02 | 14.12∗∗∗∗ ± 0.01 | 18.62∗∗ ± 0.03 | 24.54∗ ± 0.04 | 24.54∗∗ ± 0.01 | 33.88∗∗ ± 0.01 | 50.11 ± 0.01 | 58.88 ± 0.02 |
| TMX -T. suecica-W | 19.05∗∗ ± 0.03 | 26.91 ± 0.03 | 36.30 ± 0.04 | 38.90 ± 0.02 | 23.98∗∗ ± 0.02 | 30.19 ± 0.02 | 34.67 ± 0.02 | 43.65 ± 0.01 | 46.77∗ ± 0.02 | 56.23 ± 0.01 | 70.79 ± 0.02 | 85.11 ± 0.02 |
| TMX -T. suecica-CHL |
27.54∗ ± 0.01 |
33.11 ± 0.01 |
37.15 ± 0.01 |
43.65 ± 0.01 |
14.12∗∗∗ ± 0.01 |
15.13∗∗ ± 0.01 |
16.98∗∗ ± 0.03 |
28.18 ± 0.03 |
30.19∗∗ ± 0.01 |
44.66 ± 0.01 |
48.97 ± 0.01 |
66.06 ± 0.02 |
| TMX -Chlorella sp.-ETH | 22.38∗∗ ± 0.02 | 26.91 ± 0.02 | 38.9 ± 0.04 | 52.48 ± 0.02 | 16.98∗∗ ± 0.01 | 20.41∗ ± 0.01 | 21.37 ± 0.03 | 28.84 ± 0.06 | 30.19∗∗ ± 0.02 | 38.9 ± 0.01 | 56.23 ± 0.02 | 70.79 ± 0.02 |
| TMX -Chlorella sp.-W | 24.54∗ ± 0.04 | 29.51 ± 0.04 | 33.88 ± 0.02 | 39.81 ± 0.04 | 21.37∗ ± 0.02 | 26.30 ± 0.01 | 38.01 ± 0.02 | 39.81 ± 0.01 | 42.65 ± 0.02 | 56.23 ± 0.01 | 63.09 ± 0.01 | 85.11 ± 0.02 |
| TMX -Chlorella sp.-CHL | 13.48∗∗∗ ± 0.02 | 21.87∗∗ ± 0.02 | 44.66 ± 0.02 | 54.95 ± 0.03 | 16.98∗∗ ± 0.01 | 18.62∗ ± 0.03 | 19.95∗ ± 0.01 | 25.11 ± 0.03 | 31.62∗ ± 0.01 | 50.11 ± 0.03 | 60.25 ± 0.02 | 66.06 ± 0.02 |
Data expressed as mean ± standard deviation (n = 3). for statistically significant difference between microalgae extracts and the ratio. Significant level: 0.010 < p ≤ 0.05, significant ∗; 0.001 < p ≤ 0.010, very significant∗∗; and p ≤ 0.001, highly significant ∗∗∗. The symbol “- “indicates no IC50 estimated.