TABLE 1.
Growth characteristics and β-galactosidase and proteinase activities of L. helveticus CRL 1062 and its slowly milk-coagulating variant S1
| Strain | Growtha
|
Sp act (mean ± SD)
|
|||
|---|---|---|---|---|---|
| RSM | RSM-G | RSM-YE | β-Galactosidaseb | Proteinasec | |
| CRL 1062 | + | + | + | 6.6 ± 0.2 | 6.1 ± 0.2 |
| S1 | − | − | + | 6.5 ± 0.3 | 6.2 ± 0.2 |
Strains were inoculated (2%) into the indicated media and incubated for 16 h at 42°C. Growth was monitored as milk coagulation (+).
Micromoles of ONP liberated per microgram of protein per minute at 42°C.
Nanomoles minute−1 milligram of protein−1. The lactate dehydrogenase activity was less than 0.06% of the total activity in each cell extract.