TABLE 2.
Maximal specific growth rate of L. helveticus CRL 1062 and S1 in SCDM supplemented with different groups of nucleotides and vitamins
| Strain | μmax (h−1) ina:
|
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|---|---|---|---|---|---|---|---|---|
| CDM | SCDM | SCDM supplemented with groupb:
|
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| A | B | C | D | E | F | |||
| CRL 1062 | 0.36 | 0.34 | 0.35 | 0.36 | 0.34 | 0.36 | 0.35 | 0.35 |
| S1 | 0.35 | 0.10 | 0.09 | 0.36 | 0.09 | 0.09 | 0.10 | 0.35 |
Values are averages from at least three independent experiments. Standard deviations were <5%.
Different pools of vitamins and nucleotides were used to characterize the specific requirements of L. helveticus S1. Group A, folic acid, vitamin B12, and thiamine; group B, adenine, inosine, and guanine; group C, orotic acid, biotin, and p-aminobenzoic acid; group D, folic acid, inosine, and orotic acid; group E, vitamin B12, adenine, and biotin; group F, thiamine, guanine, and p-aminobenzoic acid.