Table 1.

PCR Primer(s).

Gene	Sequence of primers (5’ -3’)	Product Size	Cycling parameters
TLR4	F:CTT TCA GGG AAT TAG GCT CC R: CCA AGA TCA ACC GAT GGA C	116 bp	10min at 95°C, 30sec at 95°C, 30sec at 55°C, 20 sec at 72°C, for 40 cycles
NF-κB	F:ATC TGT TTC CCC TCA TCT T R: GTG CGT CTT AGT GGT ATC TG	167 bp	10min at 95°C, 30sec at 95°C, 30sec at 55°C, 20 sec at 72°C, for 40 cycles
MIF	F:TCT CCG CCA TGC CTA TG R:GGG TCG CTC GTG CCA CTA AA	178 bp	10min at 95°C, 30sec at 95°C, 30sec at 60°C, 20 sec at 72°C, for 40 cycles
MadCAM-1	F:CCG AAA TCC ACC AGA ACC R: TCC AAT GCA CCG TCA CTC	81 bp	10min at 95°C, 30sec at 95°C, 30sec at 54°C, 20 sec at 72°C, for 40 cycles
SP-A	F:CCA GAG CAG GAG GCA ACA T R:AGA AGC CCC ATC CAG GTA G	149 bp	10min at 95°C, 30sec at 95°C, 30sec at 58°C, 20 sec at 72°C, for 40 cycles
GAPDH	F: CCA TGG AGA AGG CTG GG R: CAA AGT TGT CAT GGA TGA CC	195 bp	10min at 95°C, 30sec at 95°C, 30sec at 54°C, 20 sec at 72°C, for 40 cycles