Table 2.

Release of molecular cargo from Qβ particles assayed by dialysis against buffer. “to DNA” or “to BSA” refers to experiments in which DNA or BSA were present in the dialysis reservoir, but not in the VLP solution.

Qβ@ a	T (°C)	[X]0 (µM) b	max. release c	kout (h−1)	kin* (h−1)	k0* (µM•h−1)
TO1	25	1.83	30%	0.016	0.04	0
TO2	25	3.17	27%	0.025	0.07	0
TO1	37	1.83	76%	0.04	0.0015	0
TO2	37	3.17	80%	0.04	0.0013	0
TO1 to DNA	25	1.89	51%	0.03	0.09	0.0041
TO1 to DNA	37	1.89	88%	0.045	0.025	0.0038
TO1 to BSA	37	1.56	100%	0.09	0	0
TO-PEG81	25	0.90	79%	0.052	0.042	0.0030
TO-PEG82	25	1.21	77%	0.050	0.055	0.0052
TO-PEG810	25	1.85	79%	0.060	0.048	0.0060
TO-PEG81	37	0.90	90%	0.15	0.026	0
TO-PEG82	37	1.21	88%	0.125	0.02	0
TO-PEG810	37	1.85	92%	0.11	0.014	0
TO-PEG451	37	0.052	31%	0.1	0.22	0
TO-PEG4510	37	0.341	25%	0.055	0.18	0
TO-PEP180.5	37	0.207	92%	0.165	0.13	0.0025
TO-PEP190.5	37	0.259	90%	0.13	0.18	0.0034
TO-PEP290.5	37	0.234	87%	0.06	0.009	0

^[a]Cargo molecule packaged inside VLP.

^[b]Starting concentration of cargo, calculated as though the packaged molecules were free in solution.

^[c]Percentage of starting cargo released at the end of the experiment (after 100–150 h).