Table 3.

Packaging of TO-peptide molecules. N = average number of molecules bound per particle; EE = entrapment efficiency; LE = loading efficiency; incubation time before sucrose gradient purification = 12 h in all cases; [Qβ] for all experiments = 1.25 mg/mL. Error is standard deviation for at least three independent replicates. The values for pI and charge are calculated predictions, not experimentally determined.

cargo	MW	water soluble?	conc (mM) a	N	EE	LE
TO-Pep18	2462	Yes	0.15	176 ± 29	58%	17%
TO-Pep18			0.50	508 ± 47	50%	42%
TO-Pep19	2538	sparingly	0.15	122 ± 32	40%	12%
TO-Pep19			0.50	432 ± 65	43%	36%
TO-Pep29	3275	Yes	0.15	132 ± 23	40%	15%
TO-Pep29			0.50	346 ± 51	34%	45%

Pep₁₈ = Pra-GSVGGNYNYLYRLFRKS; pI = 10.3, +3.0 at neutral pH

Pep₁₉ = Pra-GSATRFASVYAWNRKRIS; pI = 12.2, +4.0 at neutral pH

Pep₂₉ = Pra-GSGSDGSPLEFISQAVHAAHAEINEAGR; pI = 12.2, −2.8 at neutral pH