Table 1.
Framework of experimental design.
| Time | ||||
|---|---|---|---|---|
| Latency period 18 h | ||||
| 5 pm | 5 pm | 5 am | 11 am | |
| Treatments | 1st injection | 2nd injection* | 3rd injection | Stripping** |
| N | Negative control (PBS) |
x | x | – |
| P | Positive control (OvatideTM) |
x | x | – |
| A | OvatideTM | x | Isotocin_12pi | + |
| B | OvatideTM | x | Isotocin_vasotocin-12pi | + |
| C | OvatideTM | Isotocin_1 | x | + |
| D | OvatideTM | Isotocin_vasotocin_1 | x | + |
| E | OvatideTM | CNT_Isotocin_1 | x | + |
| F | OvatideTM | CNT_Isotocin_Vasotocin_1 | x | + |
*1st and 2nd injection-Single time * Second injection- after 12 h.
(X) No injection.
(-) Not response on stripping.
(+) Response on stripping.
**Stripping: after 18 h.