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. Author manuscript; available in PMC: 2023 Jul 11.
Published in final edited form as: Cancer Cell. 2022 Jun 2;40(7):720–737.e5. doi: 10.1016/j.ccell.2022.05.006

Figure 1: Exercise restricts pancreatic cancer growth and reprograms tumor immunity.

Figure 1:

a. The forced treadmill running model (exercise) is shown. b. 8-week old KC mice were exercised for 6 weeks or remained sedentary. Pancreatic tissue sections stained with Hematoxylin and Eosin (left) and Trichrome and Gomori (right) at 14 weeks of age are shown, with quantification of % acinar and %fibrotic area/field of view (FOV). Each dot = one mouse; n = 4–5. c. 8-week old female C57BL/6J wild type (WT) mice [hereafter “WT mice”] were injected orthotopically with 1×10^5 KPC 4662 cells into the pancreas at Day 0 [hereafter “orthotopically injected”] and exercise was started at Day 1. Day 21 tumor weights and images are shown. Each dot = one tumor (n = 7). Experiment was repeated ten times. d. WT mice were injected orthotopically with 5×10^4 KPC cells and began exercise at Day 12. Schema, tumor images, and weights are shown. Each dot = one tumor (n = 9–10). Experiment was repeated three times. (e-f) WT mice were injected orthotopically and exercise was started at Day 1. e. Single cell RNASeq (sc-RNAseq) was performed on all live leukocytes (PI-, CD45+) from Day 21 tumors. tSNE plots show distribution of clusters, identified by distinct colors (n = 3 tumors pooled / group). f. Relative fraction of each cluster in control and exercise. (p < 0.05 = *, p < 0.01 = **). See Supp. 1