Key resources table for Kurz et. al.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| PE anti-mouse CD125 (IL-15Rα) (clone 6B4C88) | Biolegend | Cat #: 153503 |
| IL15RA Polyclonal Antibody | Invitrogen | Cat #: PA5 – 79467 |
| In VivoMAb anti-mouse PD-1 (clone RMP1-14) | BioXcell | Cat #: BE0146 |
| In VivoMAb Anti-mouse IL-15 (clone AIO.3) | BioXcell | Cat #: BE0315 |
| In VivoMAb Anti-mouse CD8 alpha (clone 2.43) | BioXcell | Cat #: BE0061 |
| PE/Cyanine7 anti-mouse CD8 alpha (clone 53–6.7) | Biolegend | Cat #: 100721 |
| APC anti-mouse CD279 (PD-1) (clone 29F.A12) | Biolegend | Cat #: 135209 |
| Brilliant Violent 421™ anti-mouse CD3 (clone 17A2) | Biolegend | Cat #: 100227 |
| PerCP/Cyanine5.5 anti-mouse IFN-Y (clone XMG1.2) | Biolegend | Cat #: 505821 |
| Goat Anti-Rabbit IgG H&L (AF488) | Abcam | Cat #: ab150077 |
| Recombinant anti-IL-15 antibody [EPR22635–214] | Abcam | Cat #: ab273631 |
| PE/Dazzle™ 594 anti-T-bet (clone 4B10) | Biolegend | Cat #: 644827 |
| PerCP/Cyanine5.5 anti-human/mouse Granzyme B Recombinant Antibody (clone QA16A02) | Biolegend | Cat #: 372211 |
| PE/Dazzle™ 594 anti-mouse Ki67 (clone 16A8) | Biolegend | Cat #: 652427 |
| Alexa Flour 647 anti-TCF1 (TCF7) (clone 7F11A10) | Biolegend | Cat #: 655203 |
| Mouse anti-Human CD8 Ab-1 (Clone C8/144B) | Thermofisher | Cat #: MS-457-S |
| Mouse anti-Human GranzymeB (clone 11F1) | Leica Biosystems | Cat #: GRAN-B-L-CE |
| Brilliant Violent 421™ anti-mouse/human CD44 (clone IM7) | Biolegend | Cat #: 103039 |
| Bacterial and virus strains | ||
| Biological samples | ||
| Human Pancreatic Cancer Surgical Specimens from Exercised Patients | University of Texas MD Anderson Cancer Center | https://clinicaltrials.gov/ct2/show/NCT03187951 |
| Human Pancreatic Cancer Surgical Specimens from Historical Control Cohort | University of Texas MD Anderson Cancer Center | https://www.nature.com/articles/s41598-019-49582-3 |
| Chemicals, peptides, and recombinant proteins | ||
| Recombinant human IL-15 Superagonist (IL-15 S.A.), hetero-dimer of human IL-15 and sIL-15Ra made in HEK293 Cells | Novartis, via MTA with NYU Langone | NIZ985 |
| Fingolimod hydrochloride (FTY720) > 98% HPLC | Sigma Aldrich/Millipore | Cat #: SML0700, CAS Number: 162359-56-0 |
| (−)-Epinephrine | Sigma Aldrich | Cat #: E4250, CAS Number: 51-43-4. EC Number: 200-098-7 |
| (±)-Propranolol hydrochloride, > 99% TLC, powder | Sigma Aldrich | Cat #: P0884, CAS Number: 318-98-9. EC Number: 206-268-7 |
| Critical commercial assays | ||
| Epinephrine ELISA Kit (Colorimetric) | Novus Biologicals | Cat #: KA1882 |
| Mouse IL-15 ELISA Kit | Sigma-Aldrich | Cat #: RAB0260 |
| Deposited data | ||
| 10x Single Cell RNA Sequencing Raw Data | This paper | GEO: GSE176311 |
| Experimental models: Cell lines | ||
| Mouse: KPC 4662 Pancreatic Cancer cell line Derived from LSL-KRASG12D/+ p53R172H/+; Pdx-1Cre/+ mice |
Gift from Robert Vonderheide, UPENN | N/A |
| Mouse: KPC 1203 Pancreatic Cancer cell line Derived from LSL-KRASG12D/+ p53R172H/+; Pdx-1Cre/+ mice |
NYU Grossman School of Medicine, Department of Cell Biology | N/A |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J wild type mice | The Jackson Laboratory | JAX: 000664 |
| Mouse: KC LSL-KRASG12D/+; p48Cre/+ | NYU Langone Medical Center, Division of Comparative Medicine mouse facility | N/A |
| Mouse: NU/J Foxn1nu athymic nude mice | The Jackson Laboratory | JAX: 002019; RRID: ISMR_JAX:002019 |
| Mouse: B6.129S7-Rag1tm1Mom/J Rag1KO mice | The Jackson Laboratory | JAX: 002216, RRID: IMSR_JAX:002216 |
| Oligonucleotides | ||
| Recombinant DNA | ||
| Software and algorithms | ||
| GraphPad Prism 9.3.1 | Dotmatics | https://www.graphpad.com |
| iCellR R package (v1.5.5) | This paper | https://CRAN.R-project.org/package=iCellR |
| Ingenuity Pathway Analysis (IPA) | Qiagen | https://digitalinsights.qiagen.com/products-overview/discovery-insights-portfolio/analysis-and-visualization/qiagen-ipa/?gclid=Cj0KCQjwpcOTBhCZARIsAEAYLuU2uBoPYEwAjfKm5S0IeMMKdT2e6A9WQDKXBNipkzh0QNE9wNgH-mQaAspZEALw_wcB |
| FlowJo Version 10.6.1 | Treestar | https://www.flowjo.com/solutions/flowjo |
| inform Tissue Analysis Software | Akoya Biosciences | https://www.akoyabio.com/phenoimager/software/inform-tissue-finder/ |
| Vectra Polaris Multispectral Imaging System | Akoya Biosciences | https://www.akoyabio.com/phenoimager/instruments/vectra-3-0/ |
| Other | ||
| Rodent Five-Lane Treadmill | Harvard Apparatus | Cat #: 76–0895 |