Figure 2.
Overview of the NI01 peptide series and the observed dual mechanism against anionic lipid bilayers.34 (A) Sequences of the epidermicin NI01 primary structure, its 2-helix substructure derivatives, and the arginine mutant R-NI01. The three substructures are notated as (α1α2, α2α3, and α3α4). The four helices are color coded above the sequences and labeled α1−α4 accordingly. Turns are underlined in the sequences. (B) Crystal structure of NI01. Ribbon representation from the N terminus (green) to the C terminus (orange). (C) In-liquid AFM images representing the topography of NI01-treated PC/PG (3:1) SLBs mimicking bacterial membranes. The floral-shaped topography consists of bilayer spanning petal-shaped pores (dark regions) and a membrane thinning patch in the center (light regions).