TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype or phenotype | Source or reference |
|---|---|---|
| P. aeruginosa strains | ||
| PAO1 | Wild type | 13 |
| PAO-JP1 | ΔlasI::Tet strain PAO1 derivative | 19 |
| PDO-100 | ΔrhlI::Tn501-2 strain PAO1 derivative | 3 |
| PAO-JP2 | ΔlasI::Tet ΔrhlI::Tn501-2 strain PAO1 derivative | 19 |
| Plasmids | ||
| pFPV25 | Promoterless GFPmut3 transcriptional fusion vector, Ampr | 5 |
| pUCP22 | Broad-host-range cloning vector, Ampr | 30 |
| pTdK-GFP | GFPmut3.1 gene under control of the lac promoter, contains an origin of replication for both P. aeruginosa and E. coli, Ampr | This study |
| pJK1 | Promoterless GFPmut3.1 transcriptional fusion vector, contains an origin of replication for both P. aeruginosa and E. coli Ampr | Our laboratory |
| pJBA111 | Source of the LVAgfp gene | 1 |
| pLPRI | rhlI-lacZ transcriptional fusion, Ampr | 29 |
| p116>97Mn#1 | lasI-lasZ transcriptional fusion (with an A-to-G substitution 36 bp upstream of the lasI start of translation, Ampr | Our laboratory |
| pTdK-LVAgfp | Promoterless LVA-gfp transcriptional fusion vector, Ampr | This study |
| plasI-LVAgfp | pTdK-LVAgfp containing an lasI-LVAgfp transcriptional fusion, Ampr | This study |
| pTdK-rhlIGFP | pJK1 containing an rhlI-gfp transcriptional fusion, Ampr | This study |
| prhlI-LVAgfp | pTdK-LVAgfp containing an rhlI- LVAgfp transcriptional fusion, Ampr | This study |