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. 2022 Jul 11;31:09636897221106998. doi: 10.1177/09636897221106998

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© The Author(s) 2022

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — M2 macrophages polarized by hypoxia/exos promote the migration, invasion, and epithelial-mesenchymal transition in A549 cells. (A) Transwell migration assay in A549 cells co-cultured with hypoxia/exos (or normoxia/exo)-treated macrophages. (B) Transwell invasion assay in A549 cells co-cultured with hypoxia/exos (or normoxia/exo)-treated macrophages. (C) Western blot analysis of EMT related proteins (E-cadherin, N-cadherin, Vimentin). M, RAW264.7 macrophages; Data represent the mean ± SD of three separate experiments; comparison was performed with Student’s t-test. Scale bar: 100 μm. *P < 0.05; **P < 0.01.