Skip to main content
. Author manuscript; available in PMC: 2023 Jun 23.
Published in final edited form as: Nat Metab. 2022 Jun 23;4(6):759–774. doi: 10.1038/s42255-022-00585-x

Extended Data Fig. 6. CCL18 and the CCL18 target GLUD1 regulate survival of tissue-infiltrating Mϕ.

Extended Data Fig. 6.

CFSE-labeled Mϕ were adoptively transferred into NSG mice that had been engrafted with human synovium. Synovial grafts were harvested after 7 days and tissue-infiltrating Mϕ were analyzed by flow cytometry. (a). Chimeric mice were treated with rhCCL18 (50 μg/mouse, n=9) or vehicle control (n=10) for 7 days. Grafts were explanted and percentages of CFSE+ Mϕ within the CD3neg cell population were measured, Data are presented as violin plots. (b). Before the adoptive transfer, Mϕ were transfected with a control (n=10), GLUD1-expressing vector (n=9). Synovial grafts were harvested and processed for flow cytometric analysis. Percentages of CFSE+ Mϕ within the CD3neg cell population were measured. Data are presented as violin plots. (c). Chimeras were reconstituted with CFSE-labeled Mϕ originating from RA patients and treated with the GLUD1 inhibitor R162 (10 mg/kg, n=12) or vehicle control (n=12). Frequencies of CFSE+ Mϕ within the CD3neg cell population were measured. Data are presented as violin plots. (D-F) Proliferation indices of Mϕ isolated from the explanted human synovial tissue were determined based on CSFE dilution. Data are in box and whisker plots.