TABLE 1.
Number of phylogenetic groups, clones, and cloned sequence types from PCB-polluted soil for each of the bacterial divisions observed in either the 16S rDNA library, the two 16S rRNA libraries, or both types of library simultaneously
| Bacterial division | No. of phylogenetic groupsa observed in PCB-polluted soil 16S rDNA and 16S rRNA libraries
|
|||||
|---|---|---|---|---|---|---|
| Total | DNA library | RNA libraries | DNA plus RNA libraries | DNA library only | RNA libraries only | |
| Proteobacteria | ||||||
| Alpha subdivision | 46 (128/99) | 20 | 40 | 14 (88/61) | 6 (6/6) | 26 (34/32) |
| Beta subdivision | 25 (124/81) | 18 | 18 | 11 (105/62) | 7 (8/8) | 7 (11/11) |
| Gamma subdivision | 10 (42/26) | 6 | 9 | 5 (35/19) | 1 (1/1) | 4 (6/6) |
| Holophaga-Acidobacterium | 23 (68/58) | 11 | 19 | 7 (41/34) | 4 (5/5) | 12 (22/19) |
| Actinobacteria | 6 (9/8) | 0 | 6 | 0 | 0 | 6 (9/8) |
| Planctomycetales | 5 (9/8) | 2 | 4 | 1 (3/3) | 1 (1/1) | 3 (5/4) |
| Candidate division OP10 | 4 (7/7) | 1 | 4 | 1 (2/2) | 0 | 3 (5/5) |
| Plastids | 3 (3/3) | 2 | 1 | 0 | 2 (2/2) | 1 (1/1) |
| Low G+C gram positives | 2 (2/2) | 0 | 2 | 0 | 0 | 2 (2/2) |
| Cytophaga-Flavobacterium-Bacteroides | 1 (1/1) | 0 | 1 | 0 | 0 | 1 (1/1) |
| Not affiliated (WPS-1 and WPS-2) | 6 (11/10) | 3 | 4 | 1 (2/2) | 2 (2/2) | 3 (7/6) |
| Total | 131 (404/303) | 63 | 108 | 40 (276/183) | 23 (25/25) | 68 (103/95) |
a
Phylogenetic group: a clone group with sequence similarity higher than 97% (98% for Burkholderia and Pseudomonas-type clones). Values in parentheses indicate the number of clones/cloned sequence types.