Figure 5.

Germination rates of soybean pollen after different freezing and preservation treatments. LN → −80°C, flowers were frozen in liquid nitrogen (LN) for 72 h and then stored in a −80°C freezer; −80°C → −80°C, flowers were frozen and preserved in a −80°C freezer; LN → LN, flowers were frozen and preserved in LN. The flowers at the initially-open stage were collected and dehydrated in a drying oven at different times. After preservation, the flowers were thawed at room temperature for germination tests. All data were represented as mean ± SD of three replicates.