Figure 6.

Comparison of germination rates of preserved soybean pollen after different preservation and thawing methods. LN → −80°C, frozen in liquid nitrogen for 72 h and then stored in a −80°C freezer; −80°C → −80°C, frozen and preserved continuously in a −80°C freezer; LN → LN, frozen and preserved in liquid nitrogen. The preserved samples were thawed for 1 min by three methods. Means of germination rates of preserved pollen after different preservation and thawing methods were separated by Duncan's multiple range test at P < 0.05, where the F-test was significant.