Figure 7.

Comparison of the true hybrid rates in F1 generation of combinations using preserved and freshly collected flowers as the pollen sources. Preserved pollen: the flowers of a purple flower variety Heihe43 at the initially-open stage were collected, dehydrated, and frozen in liquid nitrogen (LN) for 72 h and then stored at −80°C, from 15 July 2018 to 24 July 2019; Fresh pollen: Fresh flowers of Heihe 43 at the initially-open stage was collected in Heihe within 0.5 h to pollinate before hybridization. Data were represented as mean ± SD of two replicates. Means of the true hybrid rates in F1 generation of combinations using preserved and freshly collected flowers as the pollen sources were separated by Duncan's multiple range test at P < 0.05, where the F-test was significant.