Figure 4.

Protein and genetic analysis of samples with different percentages of full capsids, including 75% (blue), 50% (purple), 25% (pink), and 4% (green). (a) Electropherogram representative of VP3, VP2, and VP1 capsid proteins from smallest to largest weight (left to right) of both full and empty samples. (b) Summarized integrated area under the curve of the VP protein peaks for the four samples of varying full capsid percentages. (c) Representative electropherogram of the genetic material of the four samples, where the ssDNA peak is observed to decrease as the percentage of full capsids decreases. (d) Summarized area under the curve for the genetic material peak of each sample, for all three runs the empty AAV sample did not produce a peak.