Table 2. Estimation of Sample Total Protein Concentration Based on the VP Peak Area under the Curve of the Standard (Known Concentration) and the Area under the Curve of the Sample^a.

Sample No.	Total Concentration (VP/mL)	Protein Area	Predicted Total Concentration (VP/mL)	Prediction Error Rate (%)
Set 1
1–0	1.07 × 1012	41.71 ± 1.52	Standard	Standard
1–1	1.07 × 1012	42.30 ± 3.04	1.08 × 1012	1
1–2	1.07 × 1012	43.67 ± 3.40	1.12 × 1012	5
Set 2
2–0	1.07 × 1012	39.60 ± 3.01	Standard	Standard
2–1	1.20 × 1012	41.16 ± 2.37	1.11 × 1012	7
2–2	1.33 × 1012	42.90 ± 2.68	1.12 × 1012	16
Set 3
3–0	1.07 × 1012	31.32 ± 1.68	Standard	Standard
3–1	1.07 × 1012	31.86 ± 1.08	1.08 × 1012	2
3–2	1.07 × 1012	33.52 ± 2.77	1.14 × 1012	7

^aThe predicted total concentration was estimated using eq 3. Moreover, note that each set refers to an independent run in which samples were analyzed in triplicate.