Table 3. Estimation of Sample Genomic Concentration Based on the ssDNA Area under the Curve of the Standard (Known Concentration) and the Area under the Curve of the Sample^a.

Sample No.	Genomic Concentration (GC/mL)	DNA Area	Predicted Genomic Concentration (GC/mL)	Prediction Error Rate (%)
Set 1
1-0	7.97 × 1011	11.32 ± 0.33	Standard	Standard
1-1	5.33 × 1011	8.02 ± 0.64	5.64 × 1011	6
1-2	2.66 × 1011	3.58 ± 0.52	2.52 × 1011	5
Set 2
2-0	7.97 × 1011	10.76 ± 0.32	Standard	Standard
2-1	5.38 × 1011	7.12 ± 0.70	5.27 × 1011	2
2-2	2.77 × 1011	3.64 ± 0.60	2.70 × 1011	3
Set 3
3-0	7.97 × 1011	22.47 ± 2.57	Standard	Standard
3-1	5.33 × 1011	13.55 ± 2.00	4.80 × 1011	10
3-2	2.66 × 1011	6.00 ± 1.61	2.13 × 1011	20

^aThe predicted genomic concentration was estimated using eq 4. Moreover, each set refers to an independent run in which samples were analyzed in triplicate.