Table 1. Photophysical Properties and Fluorescence Signaling Ability for RNA Sensing.

	BIOP	SYTO RNA select
emission wavelengtha	570 nm	530 nm
light-up factorb	770	500
Φboundb	0.52	0.36
εabs,maxa	2.2 × 104 cm–1 M–1	3.9 × 104 cm–1 M–1
brightness (εabs, max × Φbound)	1.1 × 104	1.4 × 104
photostabilityb	high	low

^aValues obtained in 10 mM sodium phosphate buffer solution (pH 7.0) containing 100 mM NaCl and 1.0 mM EDTA.

^b[Probe] = 1.0 μM, [E. coli total RNA] = 1.0 mM.