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. 2022 Jun 28;7(27):23127–23137. doi: 10.1021/acsomega.2c00816

Figure 2.

Figure 2

Biophysical analysis of EV samples gathered from HMC3 and C20 using P2. Panel A: CONAN assay-mediated purity assessment; mean AI (Aggregation Index) values of the EV preparations were normalized by the Aggregation Index of a solution of AuNPs in HPLC water (Norm_REF). A solution of HPLC water + AuNPs + PBS was used as negative control (intREF). See the main text for details. Panel B: TEM acquisitions were made with 25,000× zoom; bar indicates 100 nm. Panel C: DLS analysis; representative curves of the number and intensity values are shown for each sample gathered with P2. Panel D: DLS quantification of the 100K pellet gathered with P2 of each cell line; the number of EVs/uL was normalized by the number of cells from which EVs are derived to calculate the number of EVs secreted per million of cells. The data represent the mean values ± SEM of at least two different experiments. The significance of the differences was determined by t-test, *P ≤ 0.05 vs HMC3.