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. 2022 Jun 28;7(27):23127–23137. doi: 10.1021/acsomega.2c00816

Figure 3.

Figure 3

Panel A: proliferation assay of U87MG cells after administration of increasing concentrations of 2_100K EVs from HMC3 and C20. The administration was carried on for 72 h. The data were normalized to the control set to 1 and represent the mean values ± SEM of at least two independent experiments. The significance of the differences was determined by one-way ANOVA, followed by Bonferroni’s post-test: *P ≤ 0.05, **P < 0.01 vs control. Panel B: cell cycle analysis of U87MG cells treated with 100K pellet of C20 gathered with P2. The significance of the differences was determined by t-test: *P ≤ 0.05, vs the respective phase of the control. Data were collected from two independent experiments. Panel C: quantification of Cleaved/Cas3 positive U87MG cells treated with PBS (C−), 100K pellet of C20 gathered with P2, and 20 mM DTT (C+). Data were quantified by counting the cell number (n = 1929, n = 2183, n = 1694 for C–, SEV and C+, respectively) in different analyzed fields (n = 7, n = 12, n = 12 for C–, SEV and C+, respectively) from two independent experiments. The significance of the differences was determined by one-way ANOVA, followed by Bonferroni’s post-test: ****P ≤ 0.0001 vs the negative control.