TABLE 3.
Summary of mutations on the S. coelicolor rpsL or rpoB gene resulting in amino acid exchange
| Strain | Position in rpsL genea | Amino acid position (exchange) | Position in rpoB geneb | Amino acid position (exchange) | Resistance level (μg/ml)c to:
|
|||
|---|---|---|---|---|---|---|---|---|
| STRd | GEN | RIF | GNE | |||||
| 1147 | —e | 1 | 0.1 | 10 | 0.2 | |||
| S-1 | 262A→G | 88 (Lys→Glu) | 100 | 0.1 | 10 | 0.2 | ||
| S-2 | NDf | 5 | 0.2 | 10 | 0.2 | |||
| S-3 | ND | 10 | 0.1 | 5 | 0.1 | |||
| G-1 | ND | 1 | 0.3 | 10 | 0.2 | |||
| G-2 | ND | 1 | 0.3 | 10 | 0.2 | |||
| G-3 | ND | 1 | 0.3 | 10 | 0.2 | |||
| R-1 | 1049G→A | 350 (Arg→His) | 1 | 0.1 | 400 | 0.1 | ||
| R-2 | 1040A→G | 347 (His→Arg) | 1 | 0.1 | 400 | 0.2 | ||
| R-3 | 1049G→T | 350 (Arg→Phe) | 1 | 0.1 | 400 | 0.2 | ||
| SGe-1 | 262A→G | 88 (Lys→Glu) | 100 | 0.5 | ||||
| SGe-2 | 262A→G | 100 | 1 | |||||
| SGe-3 | 262A→G | 100 | 1 | |||||
| SG-1 | 262A→G | 100 | 0.3 | |||||
| SG-2 | 262A→G | 100 | 0.3 | |||||
| SG-3 | 262A→G | 100 | 0.3 | |||||
| SR-1 | 262A→G | 995T→C | 332 (Leu→Arg) | 50 | 50 | |||
| SR-2 | 262A→G | 1154C→T | 385 (Pro→Leu) | 50 | 150 | |||
| SR-3 | 262A→G | 1179C→G | 393 (Ile→Met) | 50 | 400 | |||
| SR-4 | 262A→G | 1011C→A | 337 (Asp→Glu) | 100 | 400 | |||
| SR-5 | 262A→G | 1010A→G | 337 (Asp→Gly) | 100 | 400 | |||
| SR-6 | 262A→G | 1028C→T | 343 (Ser→Leu) | 50 | 400 | |||
| SR-7 | 262A→G | 1048C→T | 350 (Arg→Cys) | 50 | 400 | |||
| SGR-1 | 262A→G | 1039C→T | 347 (His→Tyr) | 100 | 0.2 | 400 | ||
| SGR-2 | 262A→G | 1041C→A | 347 (His→Gln) | 100 | 0.2 | 400 | ||
| SGR-3 | 262A→G | ND | 50 | 0.3 | 300 | |||
a
,b Numbering originates from the start codon (GTG or ATG) of the open reading frame.
c
Determined after 4 days of cultivation on GYM agar.
d
STR, GEN, RIF, and GNE, streptomycin, gentamicin, rifampin, and geneticin, respectively.
e
—, wild-type rpsL gene.
f
Mutations were not detected (ND) within the rpsL or rpoB gene.