Fig. 4.

ZnCM NPs induced tDCs transition to inhibit CD4⁺ T cell homeostasis via OTUB1/CCL5/NF-κB. a Label-free quantitative proteomics showed the targeted protein molecules in igDCs affected by NPs. b Quantitative real-time PCR analyzing ubiquitination and deubiquitination proteasome systems of igDCs treated with NPs. c Co-Immunoprecipitation analysis investigated the binding ability of OTUB1 and CCL5. d Western blot analysis explored the regulative NF-κB, Akt and AMPK signaling in modulating CCL5 expression of igDCs treated with NPs