Fig. 7.

Arecoline treatment changes the expression of miRNAs and inflammatory factors via PI3K/AKT/mTOR pathwayin HepG2 hepatoma cells. All analyses were carried out with culture medium of HepG2 hepatoma cells treated with 2.5 μM arecoline and con group. A Exosomal miR-1267 expression. B Exosomal miR-21-3p expression. C Exosomal miR-21-5p expression. D IL-1β protein levels. E TNF-α protein levels. F Relative expression of mRNAs encoding PI3K/AKT/mTOR signaling proteins.G Western blot analysis of PI3K/AKT/mTOR signaling proteins. H Relative expression of PI3K/AKT/mTOR signaling proteins. I Western blot analysis of hub genes. J Relative expression of CDK1, CCND1, RAF1, CDKN1B and BTRC mRNA and protein. Vs CON group, ^ap < 0.05; vs 2.5 μM group, ^bp < 0.05; 5 μM group, ^cp < 0.05