Table 1.
Gene | Primer sequence (5’ → 3’) |
---|---|
AKT |
F: ACA CTC CAC TCA CTC ACA CCT CTC R: GCA CAG CCA CAC CTA CAG CAC |
PI3K |
F: CGG TTG TTA AGG AAG AGG CGA CTC R: AGT GAC TCA GGC TGG TGG ATG G |
mTOR |
F: TGG TGC TCA TTG CCT GTG CTT AG R: GGT GGC GTG AAG TGA GTC TGT G |
CDK1 |
F: CAC AAA ACT ACA GGT CAA GTG G R: GAG AAA TTT CCC GAA TTG CAG T |
CCND1 |
F: GTC CTA CTT CAA ATG TGT GCA G R: GGG ATG GTC TCC TTC ATC TTA G |
CDKN1B |
F: CTA ACT CTG AGG ACA CGC ATT T R: TTG AGT AGA ATC GTC GGT T |
RAF1 |
F: AA GAC AAG CAA CAC TAT CCG T R: CAG TAT TCC AAT CTA AGC GTG C |
BTRC |
F: CTG GAT GCC AAA TCA CTA TGT G R: GAT AAG CTT CCA CAG CAT G |
GAPDH |
F: CAG GAG GCA TTG CTG ATG AT R: GAA GGC TGG GGC TCA TTT |