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. 2022 Jul 14;159:29. doi: 10.1186/s41065-022-00241-0

Table 1.

Primer sequences used to detect target mRNAs

Gene Primer sequence (5’ → 3’)
AKT

F: ACA CTC CAC TCA CTC ACA CCT CTC

R: GCA CAG CCA CAC CTA CAG CAC

PI3K

F: CGG TTG TTA AGG AAG AGG CGA CTC

R: AGT GAC TCA GGC TGG TGG ATG G

mTOR

F: TGG TGC TCA TTG CCT GTG CTT AG

R: GGT GGC GTG AAG TGA GTC TGT G

CDK1

F: CAC AAA ACT ACA GGT CAA GTG G

R: GAG AAA TTT CCC GAA TTG CAG T

CCND1

F: GTC CTA CTT CAA ATG TGT GCA G

R: GGG ATG GTC TCC TTC ATC TTA G

CDKN1B

F: CTA ACT CTG AGG ACA CGC ATT T

R: TTG AGT AGA ATC GTC GGT T

RAF1

F: AA GAC AAG CAA CAC TAT CCG T

R: CAG TAT TCC AAT CTA AGC GTG C

BTRC

F: CTG GAT GCC AAA TCA CTA TGT G

R: GAT AAG CTT CCA CAG CAT G

GAPDH

F: CAG GAG GCA TTG CTG ATG AT

R: GAA GGC TGG GGC TCA TTT