Table 2.
Antibodies used for Western blotting
| Antibody | Manufacturer | Catalog no | Dilution |
|---|---|---|---|
| PI3 Kinase p85 (19H8) Rabbit mAb | CST | 4257 T | 1:1000 |
| Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (E3U1H) Rabbit mAb | CST | 17366S | 1:1000 |
| AKT (pan) (C67E7) Rabbit mAb | CST | 4691 T | 1:1000 |
| Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb | CST | 4060S | 1:2000 |
| mTOR (7C10) Rabbit mAb | CST | 2983 T | 1:1000 |
| Phospho-mTOR (Ser2448) Antibody | CST | 2971S | 1:1000 |
| GAPDH (D16H11) XP® Rabbit mAb | CST | 5174S | 1: 1000 |
| Anti-CDK1 (phospho T161) mAb | Abcam | ab201008-10 μl | 1:1000 |
| Anti-Cyclin D1 (CCND1) antibody | Abcam | ab40754-10 μl | 1:1000 |
| Anti-Raf1 antibody | Abcam | ab181115 | 1:1000 |
| Anti-p27 KIP 1 (CDKN1B) antibody | Abcam | ab32034 | 1:1000 |
| Anti-BTRC polyclonal Antibody | Solarbio | K002903p | 1:500 |
CST Cell Signaling Technology, mAb Monoclonal antibody