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. 2022 Jul 13;10:47. doi: 10.1186/s40364-022-00396-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — ZG16 improves the effect of chemotherapy and promotes T-cell mediated immunity a Growth curve of CT26 and CT26-ZG16 xenografts (n = 6). Data are shown as mean ± s.d.. **P < 0.01 by two-way ANOVA with Tukey’s multiple comparisons test. b the Tumor volume of CT26 and CT26-ZG16 xenografts. c H& E and IHC analysis (ZG16, CD3, PD1, PD-L1) of CT26 and CT26-ZG16 xenografts. Scale bar = 400 μm. d-e Tumor volume and growth curve of MC38 and MC38-ZG16 xenografts treated with PBS or 5-FU(n = 3). Data are shown as mean ± s.d..*P < 0.05, ***P < 0.001 by two-way ANOVA with Tukey's multiple comparisons tests. f-g Tumor volume and growth curve of MC38 xenografts treated with 5-FU, ZG16 protein or their combination (n = 3). Data are shown as mean ± s.d.***P < 0.001 by two-way ANOVA with Tukey’s multiple comparisons test