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. 2022 Jul 14;13:4075. doi: 10.1038/s41467-022-31722-5

Fig. 3. Exogenous Hedgehog ligands do not affect Th17 polarization.

Fig. 3

a Naïve CD4+ T cells were stimulated with the indicated polarizing cytokines in the presence/absence of recombinant N-terminal murine Ihh fragment at the indicated concentrations. All cells were polarized in the presence of anti-IFNγ and anti-IL4 blocking antibodies and were harvested for analysis by flow cytometry on day 5. Representative flow cytometry plots of n = 3 independent experiments are shown with a summary on the right. b Naïve CD4+ T cells were stimulated with the indicated polarizing cytokines in the presence or absence of Hh ligand blocking antibody 5E1 or isotype control antibody at 10 μg/ml. n = 4. a, b Data are means +/− SD. p-values were calculated using one-way ANOVA with Tukey's multiple comparisons test (a) or two-way ANOVA with Sidak correction (b) No statistically significant differences were observed. Source data are provided in the source data file.