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. 2022 Jul 14;13:4075. doi: 10.1038/s41467-022-31722-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a C57BL/6 mice were injected i.p. with 20 µg anti-CD3 monoclonal antibody (Clone: 145-2C11) at 0 and 48 h and dosed every 12 h by oral gavage with 100 mg/kg vismodegib or carrier control. Mice were harvested at 52 h. b Weight loss during the course of the experiment, relative to starting weight on day 0 (=100%). Pooled data from three independent experiments. c Small Intestine weight/length ratios. Representative data shown from one of three independent experiments. d IEL Th17 and IFNγ⁺ IL-17a⁻ CD4⁺ T cell numbers. Representative data shown from one of three independent experiments. Gating strategy is shown in Supplementary Fig. 8. e Serum IL-17a concentrations at 52 h. Pooled data from three independent experiments. f Rag2^−/− mice were injected i.p. with 4 × 10⁵ CD45RB^hi CD25^neg tdTom⁺ CD4⁺ T cells isolated from the spleens and peripheral lymph nodes of tamoxifen-treated CD4CreER^T2Cre Ihh^fl/+ tdTom⁺ (HET) or CD4CreER ^T2 Ihh^fl/fl tdTom⁺ (KO) mice. Mice were harvested at 6 weeks. g–j Pooled data from three independent experiments shown. g Weight loss during the course of the experiment , relative to starting weight on day 0 (=100%). h Colon weight/length ratios. (i) Numbers of IL-17a⁺, IL-22⁺, IL-17a⁺ IL-22⁺, IFNγ⁺ IL-17a^neg T cells isolated from colonic lamina propria. Gating strategy is shown in Supplementary Fig. 9. j Panels on the top show representative H&E staining of the recipient mouse colons. The panel on the bottom shows the quantification of histological severity scored blindly by a gastroenterologist. Scale bars: 200 μm. Data are means +/− SEM (b, g). Rest are means +/− SD. p-values were calculated using an unpaired two-tailed Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001. p-values were calculated for panels (c, d, e, i) using an unpaired two-tailed Student’s t test, j using a Kruskal–Wallis test, h a one-way ANOVA, or b, g a two-way ANOVA with Sidak correction. *p < 0.05, **p < 0.01, ***p < 0.001. ns = not statistically significant. Source data are provided in the source data file.