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. 2022 Jun 24;7(27):23815–23820. doi: 10.1021/acsomega.2c02482

Table 2. Validation Parameters of the Developed TLC Visualization Method to Determine LOP and BIS in their Pure Form.

parameters LOP BIS
concentration range (μg/mL) 2.00–10.00 1.00–10.00
linearity (regression equation) Y = −2.815x + 52.85 Y = −3.6595x + 47.673
correlation coefficienta 0.9999 0.9996
accuracy (mean ± SD)b 100.04 ± 0.48 99.93 ± 1.46
LOD (μg/mL)c 0.57 0.10
LOQ (μg/mL)c 1.73 0.30
precision (±RSD %)
Repeatability(μg/mL)d 0.76 1.78
Intermediate (μg/mL)e 1.10 1.55
a

Average of three determinations.

b

Average of 5 different concentrations of each LOP and BIS.

c

LOD is calculated via (SD of response/slope) × 3.3 and LOQ is via (SD of response/slope) × 10.

d

Interday precision; the RSD of 3 different concentrations; 4.00, 6.00, and 8.00 μg/mL for LOP and 2.00, 4.00, and 6.00 μg/mL for BIS; 3 replicates each; on the same day.

e

Interday precision; the RSD of 3 different concentrations; 4.00, 6.00, and 8.00 μg/mL for LOP and 2.00, 4.00, and 6.00 μg/mL for BIS; 3 replicates each; on 3 successive days.