Figure 5.

B cell response within the germinal center after immunization. Lymphocytes from the draining popliteal lymph node were analyzed after three immunizations intradermal immunization with S protein alone or with adjuvants [Poly(I:C); Alum; Poly(I:C) + Alum]. Controls were performed with PBS or Poly(I:C) + Alum. (A) Number of total cells. (B) Percentage of B220⁺ cells. (C) Number of B220⁺ cells. (D) Percentage of CD38⁺GL7^- cells. (E) Number of CD38⁺GL7^- cells. (F) Percentage of RBD⁺S Ptn⁺ cells. (G) Number of RBD⁺S Ptn⁺ cells. (H) Percentage of RBD^-S Ptn⁺ cells. (I) Number of RBD^-S Ptn⁺ cells. Figure representative of 4 independent experiments and shown as mean ± S.D. and was performed by one-way ANOVA with Tukey’s post hoc test. *p<0.03, **p<0.005, ***p<0.0002, ****p<0.0001. (SEM; n=4-13).