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. 2022 Jun 30;13:884760. doi: 10.3389/fimmu.2022.884760

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Copyright © 2022 dos-Santos, Firmino-Cruz, da Fonseca-Martins, Oliveira-Maciel, Perez, Roncaglia-Pereira, Dumard, Guedes-da-Silva, Santos, Leandro, Ferreira, Guimarães-Pinto, Conde, Rodrigues, Silva, Alvim, Lima, Marsili, Abreu, Ferreira Jr., Mohana Borges, Tanuri, Souza, Rossi-Bergmann, Vale, Silva, de Oliveira, Filardy, Gomes and de Matos Guedes

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of BALF immune cell content in mice immunized with S Ptn together with different adjuvants following challenge with inactivated SARS-CoV-2 virus. BALF cells were collected 24 hours after inactivated SARS-CoV-2 (iSARS-CoV-2) virus challenge. The percentage and number of AMs, neutrophils, and T cells from naïve and mice immunized with S Ptn and different adjuvant combinations as gated on flow cytometry plots using markers including SiglecF, CD11c, CD11b, Ly6G, Ly6C, and TCRβ. Percentage of (A) AMs (CD11b⁺CD11c^-Ly6c^hiF4/80⁺), (C) Neutrophils (CD11b⁺CD11c^-Ly6c⁺F4/80^-), and (E) T cells (CD11b⁺CD11c^-Ly6c⁺F4/80^-) in the BALFs. Absolute numbers of (B) AMs, (D) Neutrophils, and (F) T cells in the BALFs. Data are presented as the mean ± SEM of three pooled experiments and analyzed by one-way ANOVA with Tukey’s post hoc test, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.