Figure 4.
Knockout mouse serotonin concentration and secretion in response to thrombin. Initial experiments were performed to determine if the knockout platelets responded differently than control platelets in control environments. The total concentration of serotonin was measured after (A) lysing the platelets with 0.5 μM HClO4, (B) incubating them in a nonstimulant Tyrode’s buffer, (C) or stimulating them with the natural stimulant thrombin. The knockout mice showed significant changes in their total concentration of serotonin (A, F(2,7) = 55.14, p ≤ 0.0001). However, the percentage of serotonin released in a resting stage (B, F(2,9) = 1.342, p = 0.3089) and upon stimulation (C, F(2,9) = 0.6464, p = 0.5466) was statistically indistinguishable for the knockout platelets compared to the control C57 mouse platelets. *p ≤ 0.05, ***p ≤ 0.001, and p ≤ 0.0001 vs indicated position. n = 4 for all conditions except for PF4 Tyrode’s condition with n = 3.