Figure 6.

Measurement of platelet adhesion in a microfluidic channel coated with endothelial cells. (A) Straight channel platelet adhesion polydimethylsiloxane-based microfluidic device coated with endothelial cells. (B) Platelets were flowed across the device, and adherent platelets were counted using bright field microscopy. The scale bar for the inset micrograph represents a length of 64 μm. Statistical analysis of adherent platelets was performed either when platelets were (C) not activated or (D) activated with 5 μM ADP, a natural platelet stimulant, before being flowed through the device. *p ≤ 0.05, **p ≤ 0.01 vs indicated position. n = 4 for all conditions except for PF4 Tyrode’s buffer condition with n = 3.