Figure 5.

Biased signaling for Gα_i1 and Gα_z activation by MT₂-Y141F^3.53. (a) Melatonin dose–response curve of Gα_i1 (left panel) and Gα_z (right panel) by WT MT₂ and MT₂-Y141F^3.53. (b) Basal Gα_i1 and Gα_z activation by MT₂-Y141^3.53 relative to WT. Statistical differences between melatonin-induced maximal responses (E_max) were assessed by comparing the best-fit values of top (E_max) (***p = 0.0006) in (a), while significance compared to WT MT₂ was assessed by a one-sample t-test and compared to 100 in (b). Each point represents the mean ± SEM of independent experiments performed in quadruplicate (distinct samples). n denotes the number of experiments performed.