(A,B) Representative immunohistochemical image of NDRG1 expression in a primary breast cancer specimens (A) and a brain metastasis (B), with cyctoplasmatic NDRG1 expression around necrotic areas and unrelated membranous expression on tumor cells (median 7.5%, range 0–100%, n=74). Arrows; magnification x100 and x400; scale bar 200 μm. (C) Median NDRG1 expression in primary breast tumors of patients experiencing distant metastasis (n=35) compared to patients without distant metastases (n=39) during a follow up period of 10 years (p<0.05; Mann Whitney U test). (D) Metastasis-free survival in patients with high NDRG1 expression (n=20) in the primary breast cancer specimen, compared to patients with absent or low NDRG1 expression (n= 54; p=0.035; log rank test)