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. 2022 Apr 21;33(5):ar44. doi: 10.1091/mbc.E21-12-0611

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© 2022 Tsverov et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 5: — Systematic functional dissection of the TOR2 MAS domain. (A) TOR2 MAS domain plasmid pPL626containing the S1972R (RapR) TOR1-1 allele was modified to create the indicated TOR1-TOR2 chimeras. Each construct, MAS-A through MAS-J, contains approximately 50 amino acids of TOR1 sequence dispersed along the length of the TOR2 MAS domain. Plasmids were introduced into heterozygous TOR2/tor2Δ cells and analyzed by tetrad analysis as described in the legend to Figure 3A. (B) Western blot analysis of TOR1-TOR2 chimeras. TOR2/tor2Δ cells carrying the indicated plasmids were prepared and analyzed as described in the legend to Figure 2D. (C) TOR2/tor2Δ cells carrying the indicated plasmids were tested for rapamycin resistance as described in the legend to Figure 3B.