FIGURE 1:

NC MTs polymerize into FAs and reduce FA size. (A) HUVEC expressing mApple-Golgi and GFP-CAMSAP2 showing CAMSAP2 localization in conditions of centrosomal (top row) or NC (bottom row) MTs. (B, C) Quantification of CAMSAP2 stretch length (B) and count (C). (D) HUVECs expressing mCherry-F-Tractin and GFP-EB3 and white paxillin in control (all MTs; top row), centrosomal MT (middle row), and NC MT (bottom row) cells. Yellow boxes in overlay images indicate the zoom regions shown to the right. Zoom regions show MT organization (yellow arrows in green EB max-intensity projections) relative to FAs (white) and actin organization (red) relative to the same FAs (far right panels). (E) Representative kymographs of GFP-EB3 and FA dynamics (paxillin; white) in cells with centrosomal MTs (left) or NC MTs (right; related to Supplemental Video 1 and 2). (F) Quantification of the percent change in EB growth into FAs. (G–I) Comparison of FA size (G), MT growth speed (H), and MT growth lifetime (I) in HUVECs with control, centrosomal, or NC MTs. Error bars show ±SD in B and C and ±SE in H and I. Scale bars = 10 µm, whole cell view; 1 µm, zoomed view. Student’s t test; p (*) < 0.05.