FIG. 3.

Alignment of the putative reductase (PaaE) proteins from IMB-1 and H. chloromethanicum CM2 with related reductase sequences. The putative reductase (Orf364) from IMB-1 and the partial putative reductase (Orf 240) from H. chloromethanicum CM2 have been aligned with OphA1 (phthalate dioxygenase reductase) from B. cepacia (AF095748), VanB from Pseudomonas sp. strain HR199 (Y11521), TsaB (toluenesulfonate methyl-monooxygenase reductase) from Comamonas testosteroni (U32622), and PaaE from E. coli (X97452). Similar residues are shaded in gray; identical residues are in black boxes. The FMN/FAD, NAD, and [2Fe-2S] ferredoxin conserved binding sites are indicated underneath the sequence, and other conserved residues are indicated by bullets (●) (3, 5).