Fig. 3.

Engagement of target organs in vivo. (A) Immunoblots portray the amounts of Yap phosphorylated at residue S127 (pYap) and the total amounts of Yap (tYap) in the liver, heart, and skin. Injection of TDI-011536 at a dosage of 200 mg/kg greatly reduces the amount of pYap for at least 4 h after injection in all three organs. The levels return to control values within a day. Glyceraldehyde 3-phosphate dehydrogenase (Gapdh) is included in each instance as a loading control. (B) Two hours after injection, all three dosages of TDI-011536 largely suppress the amount of pYap. (C) By 4 h after injection, pYap has recovered following injections at 50 mg/kg. The concentration of pYap remains low in the heart and liver following injections at 100 mg/kg and 200 mg/kg, but largely recovers in the skin at the former dosage.