TABLE 2.
Biodegradation of cyclohexyl- and phenylalkanoic acids by Alcanivorax sp. strain MBIC 4326
| Source compounda | Metabolite(s) | Amt of metabolites (μmol) with time:
|
|||
|---|---|---|---|---|---|
| 8 h | 1 day | 3 days | 5 days | ||
| 4-Cyclohexanebutanoic acid | Cyclohexanecarboxylic acid | Trace | 0.13 | 0.38 | |
| Cyclohexaneacetic acid | 2.6 | 4.1 | 15 | 16 | |
| 1-Cyclohexene-1-carboxylic acid | 0.03 | 0.12 | |||
| 4-Cyclohexane-2-butenoic acid | Trace | Trace | Trace | ||
| 4-Cyclohexane-3-butenoic acid | Trace | Trace | |||
| 4-Phenylbutanoic acid | Benzoic acid | 0.05 | 0.11 | 0.25 | 0.42 |
| Phenylacetic acid | 4.3 | 10 | 26 | 28 | |
| 4-Phenyl-2-butenoic acid | 0.60 | 1.7 | 1.9 | 2.3 | |
| 4-Phenyl-3-butenoic acid | 8.9 | 17 | 25 | 29 | |
| 5-Cyclohexanepentanoic acid | Cyclohexanecarboxylic acid | 0.17 | 0.23 | 1.4 | 44 |
| Cyclohexaneacetic acid | Trace | Trace | |||
| 3-Cyclohexanepropionic acid | Trace | 0.14 | Trace | ||
| 1-Cyclohexene-1-carboxylic acid | 0.21 | 3.6 | |||
| Benzoic acid | 0.08 | ||||
| 5-Cyclohexane-2-pentenoic acid | Trace | Trace | Trace | ||
| 5-Cyclohexane-3-pentenoic acid | Trace | Trace | |||
| 5-Phenylpentanoic acid | Benzoic acid | 1.2 | 7.5 | 31 | 43 |
| Phenylacetic acid | Trace | Trace | Trace | ||
| 3-Phenylpropionic acid | 5.2 | 21 | 4.5 | 0.25 | |
| trans-Cinnamic acid | 3.2 | 14 | 2.9 | 0.08 | |
| 5-Phenyl-2-pentenoic acid | Trace | Trace | Trace | ||
| 5-Phenyl-3-pentenoic acid | Trace | Trace | Trace | ||
| 4-Phenyl-3-butenoic acid | Benzoic acid | NDb | ND | ND | 1.8 |
| trans-Cinnamic acid | Benzoic acid | ND | ND | ND | 2.5 |
| Cyclohexanecarboxylic acid | 1-Cyclohexene-1-carboxylic acid | ND | ND | ND | 0.77 |
| Benzoic acid | ND | ND | ND | 0.06 | |
| 1-Cyclohexene-1-carboxylic acid | Benzoic acid | ND | ND | ND | 0.39 |
| 3-Cyclohexene-1-carboxylic acid | Benzoic acid | ND | ND | ND | 0.09 |
a
Substrate concentration was as follows: 1 g/liter in 10 ml of medium contains 59, 61, 54, 56, 62, 67, 78, 79, and 79 μmol of 4-cyclohexanebutanoic acid, 4-phenylbutanoic acid, 5-cyclohexanepentanoic acid, 5-phenylpentanoic acid, 4-phenyl-3-butenoic acid, trans-cinnamic acid, cyclohexanecarboxylic acid, 1-cyclohexene-1-carboxylic acid, and 3-cyclohexene-1-carboxylic acid, respectively.
b
ND, not determined.