Fig. 2.

Expression analyses of pEVTs, iEVTs, and TB-ORG-EVTs. Placenta and decidua basalis tissues (n = 3; one sample from the seventh week and two samples from the eighth week of pregnancy) were used for preparation of donor-matched pEVTs, iEVTs, and TB-ORG-EVTs. Transcript expression was analyzed by bulk RNA-seq. (A) PCA demonstrating donor-independent clustering of the three pEVT, iEVT, and TB-ORG-EVT mRNA samples, respectively. (B) Venn diagram illustrating numbers of common and uniquely expressed genes in pEVTs, iEVTs, and TB-ORG-EVTs. (C) Heatmap showing expression of selected EVT markers. (D) Immunofluorescence of EVTs in serial sections of first-trimester placenta (CC) and decidua of the same patient. Representative images (eighth-week pregnancy tissues) are shown. Sections were counterstained with DAPI. CK7, cytokeratin 7; VIM, vimentin. (E) GSVA of a hand-curated secretory gene signature in pEVTs and iEVTs. Colors refer to pEVT and iEVT scores of the three different patients.