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VH W33-primed phage libraries and anti-α-gal affinity. (A) Polyclonal phage ELISA response of scFv libraries containing VH position 33 fixed to alanine or tryptophan through two rounds of selection. (B) The binding capacity of three VH W33 containing anti-α-gal binding clones (B9, F2, and D11), could be abolished by a heavy-chain W33A mutation. OD450 nm, optical density at 450 nm.
