Fig 2. Examples of competition between Tsukamurella paurometabola and other bacterial isolates of the collection.
Co-culture of isolate #25-T. paurometabola DSM 20162T was performed according to the arrangement illustrated in the centre, as follows: each bacterial isolate was initially inoculated at diametrically opposite parts of a 60 x 15 mm Nutrient Agar (NA) plate. T. paurometabola was inoculated along the lines labelled T1, while the second bacterium was inoculated along the lines labelled B1. The two isolates were incubated until growth was readily visible. Subsequently, a second round of inoculation took place, with T. paurometabola inoculated along the line labelled T2 and the other bacterium inoculated along the line labelled B2. The approximate distance between those inoculated lines is indicated. Visual inspection of co-cultures took place daily, over a period of 10 d. Inoculation was performed using a sterile loop twice, and the isolates were spread along the lines in the direction indicated by the horizontal arrows. Representative results of this procedure are presented, as follows: clockwise, binary co-cultures with isolates #84-Streptomyces griseus subsp. griseus FBUA 801 (a), #87-Streptomyces sp. FBUA 1287 (b), #89-Streptomyces zaomyceticus FBUA 1571 (c), #26-Lysinibacillus sphaericus ZK38 (d), #30-Bacillus licheniformis B3 (e), #35-Bacillus siamensis D9 (f), #16- Sphingomonas sanxanigenens DSM 19645T (g), #53-Hydrogenophaga carboriunda TV-122 (h) and #74-Rhodanobacter lindaniclasticus RB3-4A (i). The photographs were taken 7 d after incubation of the co-cultures and the black arrows indicate the inoculation line of the inhibited isolate. T. paurometabola is inhibited by the Actinobacteria (a-c) and the Firmicutes (d-f), but inhibits the Proteobacteria (g-i).