Figure 6.

Differential HDX between wild-type and D76N β₂m. (a) Sequence coverage map of the pepsin digest of HDX samples. (b) PAVED⁸ plot for the 30 s incubation time-point showing combined relative fractional uptake for wild-type β₂m (blue, set to 0 for reference) and the D76N variant (red). Shaded regions show combined standard deviation, n = 5. (c) Uptake plots for four peptides covering different regions of β₂m (numbered 1–4, positions in the protein sequence are as annotated in the PAVED plot in Figure 6b). Residues covered by each peptide are annotated in the top left-hand corner of each plot. Error bars show standard deviation, n = 5. (d) Structural heat map of differential hydrogen exchange. Black regions indicate the absence of data. Only statistically significant differences are shown (p < 0.05 based PAVED algorithm⁸) with an increase in deuterium incorporation for D76N β₂m shown in red. PDB: 4FXL.³³