FIGURE 1.

MAPK15 regulates ATP production rates. In all experiments, HeLa cells were transfected with scrambled siRNA or two different siRNA against MAPK15 (#1 and #2). After 24 h, they were also transfected with MYC‐PRKN and after additional 48 h, we proceeded to analysis. (a) Analysis of ATP production. Comparison of mitochondrial ATP (mitoATP) production rate and glycolytic ATP (glycoATP) production rate at basal level. (b) Analysis of oxygen consumption rate (OCR) was performed upon subsequent additions of oligomycin, FCCP and the respiratory complex I and III inhibitors rotenone and antimycin A, as indicated. (c) Analysis of the extracellular acidification rate (ECAR) with glycolysis stress test. Subsequent additions of glucose, the ATP synthase inhibitor oligomycin, and the hexokinase inhibitor 2‐deoxy‐glucose (2‐DG) were carried out as indicated. One experiment, representative of 3 independent experiments, is shown